York University Phenology Project - ITS2, library #2, >0.99 barcode matches

Occurrence
最新版本 published by Test Organization #1 on 12月 11, 2025 Test Organization #1
發布日期:
2025年12月11日
Published by:
Test Organization #1
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說明

The York University Phenology Project evaluated the utility of managed honey bee (Apis mellifera) colonies as bio-monitors of plant community phenology. Using Amro Zayed's urban apiary as the study site, pollen traps were installed at the base of 5 honey bee colonies, and pollen was collected weekly during a 24 hr. sampling period for 15 weeks. On-site field monitoring of plants in flowering was simultaneously completed using a 1000 m. radius around the study site. Collected pollen samples were split into two libraries, each of which underwent two-locus metabarcoding using ITS2 and rbcL1, yielding 4 total metabarcoding libraries. This metabarcoding library contains ITS2 ASVs for weeks 5 – 15 that were matched to a MetaCurator reference barcode with a >0.99 similarity threshold. Metabarcoding methods were independently validated and are described here: https://link.springer.com/article/10.1186/s13007-023-01097-9 The study site was urban, featuring many ornamental plantings of non-native species, and mixed-wood lots of native and invasive species.[This dataset was processed using the GBIF Metabarcoding Data Toolkit.]

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關鍵字

Occurrence

聯絡資訊

Sydney Wizenberg
  • 元數據提供者 ●
  • 出處 ●
  • 連絡人
  • Postdoctoral Fellow
Naturalis Biodiversity Center
Leiden
NL
Amro Zayed
  • 出處
  • Professor
York University
Toronto
Ontario
CA
Mateus Pepinelli
  • 出處
  • Professor
Laurentian University
Sudbury
Ontario
CA
Ida Conflitti
  • 出處
  • Project Manager
York University
Toronto
Ontario
CA

額外的詮釋資料