York University Phenology Project - ITS2, library #2, >0.99 barcode matches

Occurrence
Latest version published by Test Organization #1 on Dec 11, 2025 Test Organization #1
Publication date:
December 11, 2025
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Test Organization #1
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Description

The York University Phenology Project evaluated the utility of managed honey bee (Apis mellifera) colonies as bio-monitors of plant community phenology. Using Amro Zayed's urban apiary as the study site, pollen traps were installed at the base of 5 honey bee colonies, and pollen was collected weekly during a 24 hr. sampling period for 15 weeks. On-site field monitoring of plants in flowering was simultaneously completed using a 1000 m. radius around the study site. Collected pollen samples were split into two libraries, each of which underwent two-locus metabarcoding using ITS2 and rbcL1, yielding 4 total metabarcoding libraries. This metabarcoding library contains ITS2 ASVs for weeks 5 – 15 that were matched to a MetaCurator reference barcode with a >0.99 similarity threshold. Metabarcoding methods were independently validated and are described here: https://link.springer.com/article/10.1186/s13007-023-01097-9 The study site was urban, featuring many ornamental plantings of non-native species, and mixed-wood lots of native and invasive species.[This dataset was processed using the GBIF Metabarcoding Data Toolkit.]

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The publisher and rights holder of this work is Test Organization #1. This work is licensed under a <a href="https://creativecommons.org/publicdomain/zero/1.0/">https://creativecommons.org/publicdomain/zero/1.0/</a>.

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Keywords

Occurrence

Contacts

Sydney Wizenberg
  • Metadata Provider ●
  • Originator ●
  • Point Of Contact
  • Postdoctoral Fellow
Naturalis Biodiversity Center
Leiden
NL
Amro Zayed
  • Originator
  • Professor
York University
Toronto
Ontario
CA
Mateus Pepinelli
  • Originator
  • Professor
Laurentian University
Sudbury
Ontario
CA
Ida Conflitti
  • Originator
  • Project Manager
York University
Toronto
Ontario
CA

Additional Metadata